attune flow cytometer - An Overview

attune flow cytometer - An Overview

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Data-enriched characterization of mobile populations. Morphological information from images can incorporate to the richness of flow cytometry info. As an example, the determine reveals an usually common apoptosis assay working with Annexin V and PI, adding mobile imaging to characterize cells in Every single inhabitants to reveal morphologically unique characteristics. These insights could not are actually attained from multiplex staining by itself.

As samples are obtained over the Attune CytPix Flow Cytometer, the significant-speed brightfield digicam captures and stores visuals of detected activities in a level around six,000 photos for every 2nd, dependant upon the flow charge and graphic sizing. For better overall flexibility, Attune Cytometric Software package enables you to change the image seize frequency as essential and choose a particular gate for image seize, if needed.

(B) RAW 264.7 macrophages in many cases are annotated inaccurately with regard to particle count because of their complexity and noncircular borders. The amount of singlets obtainable for analysis will increase once the consumer trains and employs a more precise design.

Uncover new insights into your experimental data by visualizing populations using mixed fluorescence and morphology knowledge. Our application interface wizards allow downsampling, concatenation (merged documents) and dimensionality applications which can be created into your application for an intuitive, streamlined details Evaluation working experience.

*Pricing is indicative only and is particularly issue to alter. Please Make contact with your Agilent representative with any inquiries. The knowledge set forth herein is not really an offer or maybe a determination, each of that are subject to credit score acceptance, stipulations, and availability with Agilent. Agilent Money Remedies is available in picked nations around the world.

Right here, a highly trained person has gated singlets confidently. Just after assessing the handbook singlet gate, the CytPix impression-derived parameter ParticleCount reveals this gate includes greater than 4% aggregates.

Optimizing gating technique. Even sturdy guide singlet gating is error-prone and remains a subjective selection place in almost all flow cytometry assays. Imaging can be utilized to verify and adjust gates to incorporate only single cells of desire.

Our scientists will style panels for yourself as many times you would like—gratis, no products acquire wanted.

The Attune NxT cytometer would be the reputable workhorse instrument your lab can depend upon for constant and trustworthy general performance. The Attune CytPix model features a substantial-pace brightfield camera and AI-pushed image Investigation program, with new updates including machine Understanding tools and an intuitive person interface, enabling enhanced knowledge precision, precision, and quicker insights into sample biology.

Possibly most significantly, these occasions include cells of Plainly diverse phenotypes which could lead on to incorrect conclusions pertaining to double good gatherings (particularly in exceptional populations).

Seem waves help precise mobile alignment, resulting in exceptional facts high-quality and ~10X more quickly sample flow premiums.

We Formerly shown the power of imaging Vehicle-T/Ramos cell interactions. Allow’s evaluate just the population of finest desire, the double good gatherings, to learn more. We could now use extended morphological parameters (listed here, circularity and skewness of depth) to more analyze the features of those populations and refine gating on these click here occasions, increasing details robustness.

Crafted-in dimensionality reduction applications greater resolution into populations. Fluorescently gated populations back-gated on to UMAP plot to verify envisioned population resolution in lysed total human blood. Consumer can check out occasions from Each and every cluster for visual affirmation of cell phenotypes.

No wash/no lyse leukocyte Evaluation with imaging. Cells ended up acquired from 24-hour-previous blood by dilution in 1 mM EDTA ( Qualified UX/UI enables consumers to educate AI types for expanded programs. (A) The “Teach Product” function offers annotation resources and serious-time model teaching accuracy.

At the center of every Attune Flow Cytometer lies our groundbreaking acoustic focusing technology, which utilizes audio waves to meticulously align cells inside the flow mobile. This impressive strategy surpasses regular hydrodynamic methods, causing a lot quicker processing speeds, Excellent data excellent, and increased sample throughput.

As the cell line had Beforehand been analyzed to the Attune CytPix Flow Cytometer, the researchers went back again to the pictures and were being in the position to doc the microbial an infection at the least five days before.

Agilent Value Promise ensures you at the least a decade of instrument use with the day you purchase. Normally, We'll credit score you Along with the residual worth of the technique toward an upgraded model.

Right here, we display that by gating procedures using image-dependent quantitative parameters can distinguish interacting cells from coincident activities to a lot more properly analyze interacting cells.